Structural Genomics of Mouse Macrophage Proteins

Nathan Cowieson,^a Pawel Listwan,^b Christine Wells,^a Thomas Huber,^c Timothy Ravasi,^a Anna Aagaard,^a Bostjan Kobe,^b David Hume^a and Jenny Martin^a

^aIMB, University of Queensland, Brisbane, QLD, Australia; ^bBiochemistry and Molecular Biology, University of Queensland, Brisbane, QLD, Australia; ^cAdvanced Computational Modelling Center, University of Queensland, Brisbane, QLD, Australia (n.cowieson@imb.uq.edu.au)

Microarray experiments conducted in the laboratory of David Hume (IMB, UQ) have identified large numbers of genes that are transcriptionally up-regulated when mouse macrophages are challenged with various antigens. The gene products of many of these are uncharacterised. In an attempt to functionally annotate these, several structural biology groups at the University of Queensland are collaborating in a structural genomics initiative.

By gradually implementing high throughput or automated techniques to time consuming steps in the structure determination process the group aims to solve the structures, by x-ray crystallography, of as many of these novel proteins as possible. In this poster I present preliminary results on a number of these structural genomics target proteins.